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bovine Ig μ heavy-chain 1

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Depletion of conventional mature B cells and compromised specific antibody response in bovine immunoglobulin μ heavy-chain transgenic mice

Min ZHANG,Xueqian CHENG,Dan CHU,Jingwen LIANG,Yi SUN,Li MA,Beilei XU,Min ZHENG,Meili WANG,Liming REN,Xiaoxiang HU,Qingyong MENG,Ran ZHANG,Ying GUO,Yunping DAI,Robert AITKEN,Ning LI,Yaofeng ZHAO

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 2,   Pages 158-173 doi: 10.15302/J-FASE-2014015

Abstract: In this study, we introduced the bovine immunoglobulin μ heavy-chain gene (the orphaned gene on BTA11) into mouse germline cells. Bovine IgM was highly expressed in selected transgenic lines, and it largely inhibited rearrangements of the endogenous immunoglobulin heavy chain (IgH) genes in these lines. The forced expression of bovine IgM resulted in reduced numbers of pro- and pre-B cells but increased the number of immature B cells in the transgenic mice. Bovine IgM-expressing B cells can migrate from the bone marrow to the spleen, but most of the cells are arrested at the T1 transitional B cell stage, leading to a significantly lower number of T2 transitional and mature B cells in the spleen. Although the serum concentrations of endogenous IgM and IgG in the transgenic mice were significantly decreased, the IgA levels were slightly increased compared to the WT mice. The bovine IgM level in the serum was only one-tenth to one-fifth of that of endogenous mouse IgM, suggesting that most of the serum immunoglobulin were contributed by endogenous IgH gene-expressing B cells. These transgenic mice also exhibited a lower frequency of unique complementarity determining region 3 (CDR3) sequences in their VH repertoire and Vκ repertoire but exhibited an increased frequency of unique CDR3 in their Vλ repertoire. Compared to the WT mice, the transgenic mice had a significantly higher percentage of mouse IgM-expressing B cells that expressed λ chains. Finally, we showed that the transgenic mice were deficient in a specific antibody response to antigen stimulation.

Keywords： bovine Ig μ heavy-chain     transgenic mice     B cell development     allelic exclusion     immune response     Ig repertoire    

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Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion

Ruining WANG,Yubao ZHI,Junqing GUO,Qingmei LI,Li WANG,Jifei YANG,Qianyue JIN,Yinbiao WANG,Yanyan YANG,Guangxu XING,Songlin QIAO,Mengmeng ZHAO,Ruiguang DENG,Gaiping ZHANG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3,   Pages 230-236 doi: 10.15302/J-FASE-2015071

Abstract: Pure viral particles were obtained with two steps of tangential-flow filtration (TFF) and size-exclusion

Keywords： classical swine fever virus     virus purification     tangential-flow filtration     size-exclusion chromatography    

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Tetra-detector size exclusion chromatography characterization of molecular and solution properties of

Qingbin Guo,Sheng Chang

Frontiers of Environmental Science & Engineering 2017, Volume 11, Issue 2, doi: 10.1007/s11783-017-0922-x

Abstract: viscosity of SMP polysaccharides from an AnMBR were effectively determined by a high performance size exclusion

Keywords： Soluble microbial product     Extracellular polymeric substance     Membrane bioreactor     Membrane fouling     Molecular weight     Intrinsic viscosity    

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The role of natriuretic peptide precursor A gene polymorphism in the development of coronary heart disease in Chinese Han population?

Ripen NSENGA MD, Longxian CHENG PhD, Mei’an HE PhD, Tangchun WU PhD,

Frontiers of Medicine 2009, Volume 3, Issue 4,   Pages 437-442 doi: 10.1007/s11684-009-0074-x

Abstract: Genotyping of SNP was performed with Taqman SNP allelic discrimination assays by means of an ABI 7900HT

Keywords： natriuretic peptide precursor A     coronary heart disease     gene polymorphism     allelic discrimination     polymorphism    

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Title Author Date Type Operation

Depletion of conventional mature B cells and compromised specific antibody response in bovine immunoglobulin μ heavy-chain transgenic mice

Min ZHANG,Xueqian CHENG,Dan CHU,Jingwen LIANG,Yi SUN,Li MA,Beilei XU,Min ZHENG,Meili WANG,Liming REN,Xiaoxiang HU,Qingyong MENG,Ran ZHANG,Ying GUO,Yunping DAI,Robert AITKEN,Ning LI,Yaofeng ZHAO

Journal Article

Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion

Ruining WANG,Yubao ZHI,Junqing GUO,Qingmei LI,Li WANG,Jifei YANG,Qianyue JIN,Yinbiao WANG,Yanyan YANG,Guangxu XING,Songlin QIAO,Mengmeng ZHAO,Ruiguang DENG,Gaiping ZHANG

Journal Article

Tetra-detector size exclusion chromatography characterization of molecular and solution properties of

Qingbin Guo,Sheng Chang

Journal Article

The role of natriuretic peptide precursor A gene polymorphism in the development of coronary heart disease in Chinese Han population?

Ripen NSENGA MD, Longxian CHENG PhD, Mei’an HE PhD, Tangchun WU PhD,

Journal Article